| 利用透性化黑曲霉细胞制备稀有人参皂苷 Rh1的方法研究 |
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| 引用本文： | 耿瑜,蒋明星,熊正红,文孟良. 利用透性化黑曲霉细胞制备稀有人参皂苷 Rh1的方法研究[J]. 延边大学学报(自然科学版), 2019, 0(2): 122-127 |
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| 作者姓名： | 耿瑜 蒋明星 熊正红 文孟良 |
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| 作者单位： | ( 1.云南大学 生命科学学院, 云南 昆明 650500; 2.昆明学院 农学与生命科学学院, 云南 昆明 650214 ) |
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| 摘 要： | 以三七的常量三醇组人参皂苷(含Re、Rg1、R1)为底物,研究了不同通透处理工艺对黑曲霉细胞转化人参皂苷Rh1的影响.实验结果表明:当通透剂为3% EDTA(质量分数),透性化处理时间为30 min(28 ℃),超声处理时间为15 min时,黑曲霉细胞的通透性最佳; 该条件下得到的黑曲霉细胞与三醇组人参皂苷底物反应30 min(45 ℃)时,Rh1的产量达到最佳.
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| 关 键 词： | 黑曲霉 透性化 1 稀有人参皂苷Rh1 |
Study on preparation of minor ginsenoside Rh_1 from permeable Aspergillus niger cells |
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| GENG Yu1,JIANG Mingxing2,XIONG Zhenghong2,WEN Mengliang1. Study on preparation of minor ginsenoside Rh_1 from permeable Aspergillus niger cells[J]. Journal of Yanbian University (Natural Science), 2019, 0(2): 122-127 |
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| Authors: | GENG Yu1 JIANG Mingxing2 XIONG Zhenghong2 WEN Mengliang1 |
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| Affiliation: | ( 1.School of Life Sciences, Yunnan University, Kunming 650500, China; 2.College of Agriculture and Life Sciences, Kunming University, Kunming 650214, China ) |
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| Abstract: | The permeation treatment of Aspergillus niger cells producing ginsenoside Rh1 by microbial transformation was studied, with constant protopanaxatriol of Panax notoginseng(including Re, Rg1 and R1)used as the substrates. The results showed that the permeability of Aspergillus niger cells was the best when the penetrating agent was 3% EDTA(mass fraction), the permeation time was 30 minutes(28 ℃)and the ultrasonic treatment was 15 minutes. Under this condition, the Rh1 production was optimum when the Aspergillus niger cells reacted with the protopanaxatriol for 30 minutes(45 ℃). |
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| Keywords: | Aspergillus nigerAspergillus niger permeabilization permeabilization 1 minor ginsenoside Rh1 |
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