| 基于微流控芯片荧光检测的LED光源自适应补偿方法 |
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| 引用本文： | 樊夏辉,刘桂礼,孔全存,李勇,王志强,钟昊,徐涛. 基于微流控芯片荧光检测的LED光源自适应补偿方法[J]. 仪表技术与传感器, 2019, 0(6): 118-122 |
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| 作者姓名： | 樊夏辉 刘桂礼 孔全存 李勇 王志强 钟昊 徐涛 |
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| 作者单位： | 北京信息科技大学仪器科学与光电工程学院;清华大学机械工程系精密/超精密制造装备及控制北京市重点实验室;清华大学天津高端装备研究院 |
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| 基金项目： | 国家自然科学基金项目(51675054);北京市自然科学基金项目(3172013);天津市自然科学基金项目(17JCZDJC32300);北京市教委科研计划项目(KM201711232005) |
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| 摘 要： | 针对微流控芯片免疫荧光检测中LED光源不稳定导致检测区和质检区荧光采集同步实时性较差的问题,提出了一种基于光源漂移特性的自适应补偿方法。通过分析光源呈负指数形式衰减的漂移特性,引入光源时变补偿因子,对光源进行自适应补偿,实现微流控芯片高精度、高稳定性检测。对比实验结果表明光源时变补偿因子具有自适应性,且经过补偿后,免疫荧光检测的分辨率达到0.01ng/mL,CV值(离散系数,coefficient of variation)提高约50%,验证了其应用可行性。
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| 关 键 词： | 微流控芯片 免疫荧光检测 漂移特性 自适应补偿 CV值 |
Adaptive Compensation Method for LED Light Source Based on Fluorescence Detection of Microfluidic Chips |
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| FAN Xia-hui,LIU Gui-li,KONG Quan-cun,LI Yong,WANG Zhi-qiang,ZHONG Hao,XU Tao. Adaptive Compensation Method for LED Light Source Based on Fluorescence Detection of Microfluidic Chips[J]. Instrument Technique and Sensor, 2019, 0(6): 118-122 |
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| Authors: | FAN Xia-hui LIU Gui-li KONG Quan-cun LI Yong WANG Zhi-qiang ZHONG Hao XU Tao |
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| Affiliation: | (School of Instrumentation Science and Opto-Electronics Engineering,Beijing Information Science and Technology University,Beijing 100192,China;Tianjin Research Institute for Advanced Equipment,Tsinghua University,Tianjin 300300,China;Department of Mechanical Engineer,Beijing Key Laboratory of Precision/Ultra-precision Manufacturing Equipment and Control,Tsinghua University,Beijing 100084,China) |
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| Abstract: | FAN Xia-hui;LIU Gui-li;KONG Quan-cun;LI Yong;WANG Zhi-qiang;ZHONG Hao;XU Tao(School of Instrumentation Science and Opto-Electronics Engineering,Beijing Information Science and Technology University,Beijing 100192,China;Tianjin Research Institute for Advanced Equipment,Tsinghua University,Tianjin 300300,China;Department of Mechanical Engineer,Beijing Key Laboratory of Precision/Ultra-precision Manufacturing Equipment and Control,Tsinghua University,Beijing 100084,China) |
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| Keywords: | microfluidic chips immunofluorescence staining drift characteristic adaptive compensation coefficient of variation |
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