Genipin交联丝素蛋白纳米纤维膜的制备与性能

利用Genipin对再生丝素蛋白进行交联改性, 并通过静电纺丝法制备交联的丝素蛋白纳米纤维膜。利用场发射扫描电镜、 红外光谱仪、 X射线衍射仪、 热重分析仪以及拉力机等对其结构与性能进行表征与测试。结果表明, 随着交联剂Genipin质量比的增加, 交联度增加, 静电纺丝素蛋白纳米纤维平均直径增大, 标准偏差增大;Genipin交联对丝素蛋白纳米纤维结晶结构影响不大, 但热性能提高;常温条件下, 随着Genipin质量比从2%提高至15%, 丝素蛋白纳米纤维膜的力学性能逐渐增强, 质量比为10%时, 其力学性能较好, 拉伸强度和断裂应变分别为19.6 MPa和5.9%;随着试验温度从40 ℃升高到200 ℃, 丝素蛋白纳米纤维膜的拉伸强度和断裂应变先增大然后减小, 当试验温度为80 ℃时, 其力学性能较好, 拉伸强度和断裂应变分别为41.6 MPa和8.6%。

Preparation and properties of Genipin cross-linked silk fibroin nanofibrous membranes

The regenerated silk fibroin(SF) was modified by Genipin cross-linking, and then was made into cross-linked nanofibrous membranes via electrospinning. The structure and properties of nanofibers were characterized and tested by use of FE-SEM, FTIR, XRD, TGA and tensile tester. The results show that the degree of cross-linking, average diameter of nanofibers and standard deviation increase with the increasing of Genipin mass ratio. And the crystal structure of SF nanofibers is not influenced by Genipin cross-linking, but thermal properties are improved. Under room temperature, the mechanical properties of SF nanofibrous membranes are enhanced with the increasing of Genipin mass ratio from 2% to 15%. And the mechanical property is better when the Genipin mass ratio is 10%, the tensile strength and breaking strain are 19.6 MPa and 5.9% respectively. The tensile strength and breaking strain of SF nanofibrous membranes first increase and then decrease with the rising of tensile temperature from 40 ℃ to 200 ℃, the mechanical property is better at 80 ℃, the tensile strength and breaking strain are 41.6 MPa and 8.6% respectively.