雌激素对卵巢切除后雌性大鼠心肌组织中雌激素受体表达的影响

目的: 探讨雌激素对卵巢切除后雌性大鼠心肌组织中雌激素受体表达的影响。方法: 雌性SD大鼠卵巢切除术(OVX)后1周, 随机分成3组:OVX+雌激素(0.15mg/kg,s.c.)、OVX+生理盐水、对照组。4 周后,Western blotting检测α和β两种雌激素受体在心肌组织中表达。结果: 卵巢切除后,血清雌激素水平与对照组(88±22 vs 403±59)pmol/L,P<0.05]相比明显下降,左心室肌中两种雌激素受体表达均下降( P<0.05)。给予雌激素后,血清雌激素水平上升为(3864±105)pmol/L,雌激素β受体表达增多(P<0.05),α受体表达下降(P<0.05)。结论: 雌激素改变卵巢切除后雌性大鼠心肌组织中雌激素受体的表达。

Effects of estrogen on estrogen receptors in myocardium of ovariecto-mized female rats

AIM: To investigate the expression of estrogen receptors in myocardium of ovariectomized fe-male rats.METHODS: One week after bilateral ovariectomy, the female SD rats were divided into 3 groups randomly :OVX +estrogen (0.15 mg/kg,s.c.) , OVX +normal sodium and control .After 4 weeks of treatment, the expression of estrogen receptors in the myocardium were evaluated by Western blotting.RESULTS: After ovariectomy, serum estrogen de-creased significantly, compared with control( 88±22 vs 403±59) pmol/L, P <0 .05], the expression of estrogen receptors decreased( P<0.05) .After estrogen replacement therapy, serum estrogen increased ( 3864±105) pmol/L], estrogen upregulated the ex-pression of ERβreceptor( P<0.05) and downregulat-ed ERαreceptor (P<0.05) in myocardium .CONCLUSION: Estrogen replacement modifies the expres-sion of estrogen receptors in myocardium of ovariecto-mized female rats.