| 中国华东汉族人群细胞色素酶 CYP3A4 新基因型的发现与序列分析 |
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| 引用本文： | 张评浒,张娟,高逸湘,钟民,尚靖,张陆勇. 中国华东汉族人群细胞色素酶 CYP3A4 新基因型的发现与序列分析[J]. 金属学报, 2008, 13(10): 1154-1157 |
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| 作者姓名： | 张评浒 张娟 高逸湘 钟民 尚靖 张陆勇 |
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| 作者单位： | 1.中国药科大学江苏省新药筛选中心, 南京 210009, 江苏;2.江苏省人民医院麻醉科,南京 210029, 江苏;3.皖南医学院附属弋矶山医院安徽省药物临床评价中心, 芜湖 241001, 安徽 |
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| 基金项目： | 中国药科大学人才引进启动项目(211077); 先声药业研究生创新基金项目(02704009) |
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| 摘 要： | 目的: 探讨中国华东地区汉族人群细胞色素P450 3A4 基因的基因型差异。方法: 采用 RT-PCR与DNA 测序技术相结合的方法对来自华东地区的6份汉族成人肝组织 P450 3A4基因的cD-NA 进行了扩增与序列分析 。结果: 本研究从6份汉族人肝组织中首次检测到了一种新的基因型,该基因型存在三处氨基酸突变和一处核苷酸缺失, 即第71位氨基酸发生V(GTG)→A(GCG) 突变;第225位氨基酸发生V(GTC)→I(AIC)的突变;第393位氨基酸发生W(TGG)→V(GTG) 突变;第671~673位核苷酸CAG发生缺失。结论: 本研究首次发现中国华东地区汉族人群存在一种CYP3A4 新基因型。
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| 关 键 词： | P450 CYP3A4 突变 基因型 |
| 收稿时间： | 2008-09-05 |
| 修稿时间： | 2008-09-22 |
Finding and analysis of CYP3A4 new genotype from Chinese Han pop-ulation in East China |
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| ZHANG Ping-hu,ZHANG Juan,GAO Yi-xiang,ZHONG Min,SHANG Jing,ZHANG Lu-yong. Finding and analysis of CYP3A4 new genotype from Chinese Han pop-ulation in East China[J]. Acta Metallurgica Sinica, 2008, 13(10): 1154-1157 |
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| Authors: | ZHANG Ping-hu ZHANG Juan GAO Yi-xiang ZHONG Min SHANG Jing ZHANG Lu-yong |
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| Affiliation: | 1.Jiangsu Center for NewDrug Screening, China Pharmaceutical University, Nanjing 210009, Jiangsu, China;2.Department of Anesthesia of the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu,China;3.Anhui Provincial Centre forDrug Clinical Evaluation, Yijishan Hospital of Wannan Medical Colleage, Wu-hu 241001, Anhui, China |
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| Abstract: | AIM: To explore the genotype differ-ence of CYP3A4 gene from Chinese Han population in East China .METHODS: The CYP3A4 gene cDNA of six samples of adult human liver tissues from Chinese Han population were amplified and identified by RT-PCR and DNA sequencing .RESULTS: One new geno-type of CYP3A4 was identified from six samples.There are three amino acids mutations, i .e .V (GTG) →A (GCG) at 71st amino acid site ;V(GTC) →I(AIC) at 225th amino acid site;W(TGG) →V(GTG) at 393rd amino acid site, and three nucleotide acids lack, i .e . CAG from 671st to 673rd nucleotide acid site. CONCLUSION: one new genotype of CYP3A4 gene is dis-covered for the first time from Chinese Han population in East China. |
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| Keywords: | P450 CYP3A4 mutation genotype |
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