灯盏细辛注射液对小鼠肝微粒体细胞色素 P450 含量的影响

目的: 研究灯盏细辛注射液对小鼠肝微粒体细胞色素P450含量的影响 。方法: 小鼠分为空白对照组 、阳性对照组(苯巴比妥钠 80 mg/kg, i .p ., 共 7 d) 、灯盏细辛注射液正常剂量组 、高剂量组(10、30 mg/kg, i .p., 共 14 d) 。用紫外分光光度法测定细胞色素 P450及其同工酶的活性 。结果: 灯盏细辛注射液两剂量组小鼠肝微粒体的蛋白含量和细胞色素 P450 含量较空白对照组增高, 对肝重指数基本无影响 ;可抑制红霉素-N-脱甲基酶(ERD) 的活性(P <0.01) , 而对氨基比林-N-脱甲基酶(AMD) 基本无影响(P >0.05) ;苯巴比妥钠组肝重指数、蛋白含量和细胞色素 P450 含量与其他各组比较均升高(P <0.05) , 对氨基比林-N-脱甲基酶 、红霉素-N-脱甲基酶活性均有诱导作用(P <0 .01) 。结论: 灯盏细辛注射液对小鼠肝微粒体蛋白、细胞色素 P450 含量有一定影响, 对CYP3A 可能有抑制作用, 对氨基比林-N-脱甲基酶活性基本无影响。

Effects of the erigeron injection on liver microsomal cytochrome P450 system in mice

AIM: To investigate the effects of eri-geron injection on liver microsomal cytochrome P450 (CYP450) system in mice .METHODS: Mice were divided into control group (CG) , positive control group, normal-dosage group (NG) and high-dosage group (HG) .The mice of control group were given sa-line only and the mice of positive control group were given Phenobarbital sodium injection (PBN) at 80 mg/kg by peritoneal injection (i .p.) , once daily for seven days.The remaining two groups were i .p.with 10 mg/kg (normal-dosage group) and 30 mg/kg (high-dosage group) erigeron injection, respectively, once daily for two weeks.Then the total content and activi-ties of CYP450 of mice were determined by spectropho-tometer .RESULTS: The study showed that the cont-ent of CYP450 and protein in mice of normal-dosage group and high-dosage group were higher than those in control group, except liver indexes.In terms of the eri-geron injection effects on Erythromycin-N-demethylase and aminopyrine-N-demethylase, the normal-dosage group and high-dosage group can decrease the activity of Erythromycin-N-demethylase (P <0.01) , but had no effect on aminopyrine-N-demethylase (P >0.05) . Phenobarbital sodium can increase the levels of liver indexes, the content of CYP450 and protein(P < 0.05) , Erythromycin-N-demethylase and aminopyrine-N-demethylase (P <0.01) .CONCLUSION: The erigeron injection had no effect on the liver indexes and the levels of aminopyrine N-demethylase in mice. However, the content of CYP450 and protein, and the levels of Erythromycin N-demethylase were affected. The erigeron injection may be an inhibitive agent to CYP3A.