人参皂苷Rg1治疗性给药的抗心肌肥厚作用

目的 观察人参皂苷Rg1 治疗性给药对缩窄腹主动脉所致大鼠左心室肥厚的影响。 方法 SD 大鼠随机分为假手术组、模型组、Rg1 (3.75,15 mg/kg) 组以及左旋精氨酸(L-arg) 200 mg kg组。后4 组用腹主动脉缩窄术(AAC) 造模, 手术3周后腹腔注射给药共4 周。给药末监测大鼠血流动力学, 称心脏质量以计算左室肥厚指标(即左室肥厚指数, LVHI =左室重 体重) 和左室重 右室重(LVW/VW), 用实时荧光定量PCR (Real-Time PCR) 法检测心房利钠因子(ANF) 和钙调神经磷酸酶(CaN) mRNA 的表达。 结果 与假手术组比较,模型组的血压、左室内收缩压、左室舒张期末压、LVHI 和LVW/RVW 均显著升高, ±dp/d tmax 降低,ANF 和CaN mRNA 表达明显上调。Rg1 和L-arg给药不影响血压及左室内收缩压, 但显著降低左心室舒张期末压(LVEDP) 而增加±dp/dtmax, 并使LVHI 和LVW/RVW 减低, ANF 和CaN mRNA 表达下调。 结论 Rg1 具有抗压力超负荷所致左心室肥厚作用, 并可能与其抑制CaN 信息通路有关。

Anti-cardiac hypertrophic effect of ginsenoside Rg1

AIM: To investigate the inhibitory effect of ginsenoside Rg1 on left ventricular hypertrophy induced by abdominal aorta coarctation in rats. METHODS: SD rats were randomly divided into five groups, four groups of them were accepted an operation of abdominal aorta coarctation to elicit left ventricular hypertrophy (LVH), the other one was sham operation group.Rg1 (3.75, 15 mg/kg) or L-arginine (L-arg, 200 mg/kg) were administered to rats in three of those four groups respectively except model group.Three weeks after operation, Rg1 or L-arg was administered for 4 weeks.At the end of administration, the hemodynamics of the rats was detected by BL-420E system, and the LVH index (left ventricular weight body weight) and left ventricular weight/right ventricular weight (LVW/RVW) were used as hypertrophic parameters; the expressions of atrial natriuretic factors (ANF) and calcineurin (CaN) mRNA were determined by iCycler iQ Real-Time Polymerase Chain Reaction (Real-Time PCR). RESULTS: Compared with those in sham operation group, the blood pressure, the left ventricular systolic pressure, left ventricular end-diastolic pressure, LVH index and LVW/RVW were significantly increased, but the ±dp/dtmax was significantly decreased, and the ANF and CaN mRNA expressions were remarkably up-regulated in model group.Rg1 (3.75, 15 mg/kg) and L-arg (200 mg/kg) had no influence on the blood pressure and the left ventricular systolic pressure, but could significantly decrease the left ventricular end-diastolic pressure and increase the ±dp/dt max, LVH index and LVW RVW, reduce the ANF and CaN mRNA expressions. CONCLUSION: Rg1 has an inhibitory effect on the left ventricular hypertrophy induced by overload pressure in rats, which may be, in partly, mediated by its inhibitory effect on CaN signaling pathway.