青蒿琥酯对盲肠结扎穿孔术脓毒症小鼠的保护作用

目的: 探讨青蒿琥酯(AS)对盲肠结扎穿孔术(CLP)脓毒症小鼠的保护作用及其可能机制。方法: 采用CLP制作小鼠脓毒症模型,术后0、4、24、48h重复肌肉注射AS,观察7 d内CLP小鼠的死亡率;检测术后4、8 h血浆脂多糖(LPS)、TNF-α、IL-6水平;术后8 h小鼠肺脏组织形态的变化。结果: AS 明显降低CLP小鼠的死亡率(P<0.05)及血浆LPS、TNF-α和IL-6水平(P<0.05或P<0.01),减轻肺组织的损伤 。结论: AS对CLP小鼠具有显著的保护作用 ,该作用可能与其降低血浆LPS水平、减少促炎细胞因子TNF-α、IL-6的释放和减轻组织脏器的损伤有关。

Protective effects of artesunate on mouse sepsis induced by cecal liga-tion and puncture

AIM: To investigate the protective ef-fects and related mechanisms of artesunate on mouse model of sepsis induced by cecal ligation and puncture(CLP).METHODS: The septic mouse models were established by CLP .Artesunate was administrated in-tramuscularly at 0, 4, 24 and 48 h after CLP .Mortali-ty of CLP mice was observed in the next 7 days.Blood samples were collected at 4,8 h after CLP and the lev-els of LPS , TNF-αand IL-6 were detected by kinetic turbidimetric limulus test or ELISA, respectively .The morphological changes of lung tissues were observed with light microscope at 8 h after CLP.RESULTS: Artesunate degraded obviously the mortality of CLP mice (P <0.05), attenuated the levels of LPS, TNF-αand IL-6 at 4, 8 h after CLP (P<0.05 or P<0.01), and the lung tissues were also less injured in artes unate treatment group .CONCLUSION :Artesu-nate has a significantly protective effect for CLP mice which may be related to attenuating the LPS level , in-hibitting the release of proinflammatory cytokines TNF-α、IL-6 and reducing organ tissue injury .