甘西鼠尾草注射液与丹参注射液对大鼠缺血再灌注损伤保护作用的比较

目的:比较甘西鼠尾草注射液与丹参注射液对大鼠缺血再灌注损伤的保护作用。方法:采用改良线栓法阻断大鼠大脑中动脉, 建立脑缺血模型, 到达再灌注时限后进行神经功能缺损评分,用试剂盒检测脑组织中NOS、NO 含量, 并计算脑组织含水量判定脑水肿情况;通过结扎双侧颈总动脉, 考察两药对脑血管通透性的影响。结果:甘西鼠尾草注射液与丹参注射液均可改善缺血再灌注损伤大鼠神经功能缺损;降低脑组织中NOS、NO 含量;降低脑血管通透性;对脑水肿也有一定的缓解作用。结论:甘西鼠尾草注射液与丹参注射液对大鼠缺血再灌注损伤均有保护作用, 小剂量丹参注射液在降低脑水肿和神经功能缺损评分方面优于小剂量甘西鼠尾草注射液;大剂量甘西鼠尾草注射液在降低脑血管通透性方面优于等剂量的丹参注射液。

Comparison between S. miltiorrhiza Bunge Injection and S. przewalskii Maxim Injection on cerebral ischemia-reperfusion injury in rats

AIM: To compare the influence of S. miltiorrhiza Bunge Injection (SMBI) and S.przewalskii Maxim Injection (SPMI) on cerebral ischemia-reperfusion injury in rats.METHODS: Focal cerebral ischemia-reperfusion injury model was made with suture-occluded method.The levels of NOS and NO in serum and brain water content were measured.The neurological deficits of rats were observed after occluding middle cerebral artery for 45 min and reperfusion for 2 hours. RESULTS: After cerebral ischemia-reperfusion, the levels of NOS and NO in serum and brain water content increased significantly.SMBI and SPMI both effectively improve neural function, reduce hydrocephalus, and reduce the levels of NOS and NO in serum in rat models.CONCLUSION: These results suggested that SMBI and SPMI had obvious protective effect on cerebral ischemia-reperfusion injury.