雷公藤红素对小鼠的免疫抑制作用及其对IL-6 mRNA 表达影响的研究

目的:研究雷公藤红素对小鼠的免疫抑制作用及对细胞因子IL-6 mRNA 表达的影响。方法:采用碳粒廓清、迟发型变态反应、血清溶血素测定和T 淋巴细胞转化实验, 观察雷公藤红素对小鼠免疫功能的影响;运用RT-PCR 半定量法研究雷公藤红素对小鼠肝脏IL-6 mRNA 表达影响。结果:雷公藤红素能抑制小鼠血清溶血素水平(400 μg/kg), 且与剂量呈依赖关系;小鼠耳廓肿胀度研究表明, 雷公藤红素能使迟发型变态反应程度减轻(400 μg/kg );雷公藤红素剂量在0.25 μg/mL 时, 可以明显抑制植物血凝素(PHA)诱导的细胞增殖;雷公藤红素800 μg/kg 能对抗CCl₄ 引起的急性肝损伤小鼠肝脏IL-6 mRNA 含量的升高。结论:雷公藤红素在一定程度上能抑制小鼠的免疫功能, 能抑制炎症细胞因子IL-6 基因的过度表达。

Effects of tripterine on the immunosuppression action and expression of IL-6 mRNA in mice

AIM:To investigate the effects of tripterine on the immunosuppression action and the over expression of IL-6 gene in mice.METHODS:The carbon particle clearance reaction test, delayed hyper-sensitivity reaction test, serum hemolysin assay and lymphocyte transfer test were applied to observe the immunosuppression action of tripterine.Mice were orally pretreated with tripterine, and the IL-6 mRNA level was determined by RT-PCR.RESULTS:The level of serum hemolsin in mice treated with triperine (400 μg/kg) was much lower than that of control group, suggesting that tripterine may suppress the level of serum hemolsin in mice in dose dependent manner.Tripterine (400 μg/kg) had an obvious effect of immunosuppression on mice cells by relieving the delayed hypersensitivity reaction, and tripterine (0.25 μg/mL) had inhibitory effects on the activation and proliferation of mice T lymphocytes stimulated by PHA.In addition, significant increase of IL-6 mRNA level induced by CCl₄ was remarkably decreased by 800 μg/kg tripterine.CONCLUSION:Tripterine suppresses the immunological function of mice to some extent and inhibits the over expression of IL-6 gene in mice liver which is related to proinflammatory cytokines.