Biosynthetic Gene Cluster for Surugamide A Encompasses an Unrelated Decapeptide,Surugamide F |
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| Authors: | Akihiro Ninomiya Prof. Yohei Katsuyama Prof. Takefumi Kuranaga Masayuki Miyazaki Dr. Yuichi Nogi Prof. Shigeru Okada Prof. Toshiyuki Wakimoto Prof. Yasuo Ohnishi Prof. Shigeki Matsunaga Prof. Kentaro Takada |
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| Affiliation: | 1. Laboratory of Aquatic Natural Products Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan;2. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan;3. Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Hokkaido, Japan;4. Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Kanagawa, Japan |
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| Abstract: | Genome mining is a powerful method for finding novel secondary metabolites. In our study on the biosynthetic gene cluster for the cyclic octapeptides surugamides A–E (inhibitors of cathepsin B), we found a putative gene cluster consisting of four successive non‐ribosomal peptide synthetase (NRPS) genes, surA, surB, surC, and surD. Prediction of amino acid sequence based on the NRPSs and gene inactivation revealed that surugamides A–E are produced by two NRPS genes, surA and surD, which were separated by two NRPS genes, surB and surC. The latter genes are responsible for the biosynthesis of an unrelated peptide, surugamide F. The pattern of intercalation observed in the sur genes is unprecedented. The structure of surugamide F, a linear decapeptide containing one 3‐amino‐2‐methylpropionic acid (AMPA) residue, was determined by spectroscopic methods and was confirmed by solid‐phase peptide synthesis. |
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| Keywords: | biosynthesis genome mining natural products peptides Surugamide |
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