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Use of Methylmalonyl‐CoA Epimerase in Enhancing Crotonase Stereoselectivity |
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| Authors: | Dr. Refaat B. Hamed Dr. J. Ruben Gomez‐Castellanos Dr. D. Sean Froese Ewelina Krysztofinska Dr. Wyatt W. Yue Prof. Christopher J. Schofield |
| Affiliation: | 1. Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Oxford, UK;2. Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut, Egypt;3. Structural Genomics Consortium, University of Oxford, Headington, UK |
| Abstract: | The use of methylmalonyl‐CoA epimerase (MCEE) to improve stereoselectivity in crotonase‐mediated biocatalysis is exemplified by the coupling of MCEE, crotonyl‐CoA carboxylase reductase and carboxymethylproline synthase in a three‐enzyme one‐pot sequential synthesis of functionalised C‐5 carboxyalkylprolines starting from crotonyl‐CoA and carbon dioxide. |
| Keywords: | antibiotics biosynthesis carbapenem crotonases dynamic kinetic discrimination enolate reactivity stereoselectivity |