Conjugation and Evaluation of Triazole‐Linked Single Guide RNA for CRISPR‐Cas9 Gene Editing |
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Authors: | Dr Kaizhang He Eldon T Chou Shawn Begay Dr Emily M Anderson Dr Anja van?Brabant?Smith |
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Affiliation: | GE Healthcare Dharmacon Inc., Lafayette, CO, USA |
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Abstract: | The CRISPR‐Cas9 gene editing system requires Cas9 endonuclease and guide RNAs (either the natural dual RNA consisting of crRNA and tracrRNA or a chimeric single guide RNA) that direct site‐specific double‐stranded DNA cleavage. This communication describes a click ligation approach that uses alkyne–azide cycloaddition to generate a triazole‐linked single guide RNA (sgRNA). The conjugated sgRNA shows efficient and comparable genome editing activity to natural dual RNA and unmodified sgRNA constructs. |
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Keywords: | conjugation CRISPR-Cas9 gene technology oligonucleotides single guide RNA |
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