Color‐Coded Super‐Resolution Small‐Molecule Imaging |
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Authors: | Dr Paolo Beuzer Dr James J La Clair Prof Hu Cang |
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Affiliation: | 1. Waitt Advanced Biophotonics Center, The Salk Institute for Biological Sciences, La Jolla, CA, USA;2. Xenobe Research Institute, San Diego, CA, USA |
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Abstract: | Although the development of super‐resolution microscopy dates back to 1994, its applications have been primarily focused on visualizing cellular structures and targets, including proteins, DNA and sugars. We now report on a system that allows both monitoring of the localization of exogenous small molecules in live cells at low resolution and subsequent super‐resolution imaging by using stochastic optical reconstruction microscopy (STORM) on fixed cells. This represents a powerful new tool to understand the dynamics of subcellular trafficking associated with the mode and mechanism of action of exogenous small molecules. |
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Keywords: | drug discovery microscopy mode of action natural products super-resolution imaging |
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