| 油菜花多糖的提取及纯化研究 |
| |
| 引用本文： | 孙汉巨,潘丽军,靳锁. 油菜花多糖的提取及纯化研究[J]. 食品科学, 2006, 27(12): 432-436 |
| |
| 作者姓名： | 孙汉巨 潘丽军 靳锁 |
| |
| 作者单位： | 合肥工业大学生物与食品工程学院； |
| |
| 基金项目： | 安徽省“十五”科技攻关计划项目（040130441） |
| |
| 摘 要： | 对油菜花多糖的提取工艺、分离纯化和分子量测定作了研究。通过单因素实验,考察了影响多糖得率的因素;通过正交试验进一步优化提取工艺条件,得出油菜花多糖最佳提取条件是液料比为40,提取温度为100℃,提取时间为1.5h,提取次数为2次。通过凝胶柱层析法对粗多糖进行进一步纯化和分子量的测定,结果表明:油菜花多糖主要含有两种组分,其分子量分别为63.51kD和49.58kD。
|
| 关 键 词： | 油菜花 多糖 提取 纯化 |
| 文章编号： | 1002-6630（2006）17-0432-05 |
| 收稿时间： | 2006-08-19 |
| 修稿时间： | 2006-08-19 |
Study on Isolation and Purification of Polysaccharides from Flower of Rape |
| |
| SUN Han-ju,PAN Li-jun,JIN Suo. Study on Isolation and Purification of Polysaccharides from Flower of Rape[J]. Food Science, 2006, 27(12): 432-436 |
| |
| Authors: | SUN Han-ju PAN Li-jun JIN Suo |
| |
| Affiliation: | College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China |
| |
| Abstract: | The extraction and purification of the rape polysaccharides were studied .The factors that affected the extracting rates were reviewed by experiments of mono-factors. Then, adopting the orthogonal test, the optimum extraction conditions were obtained. The ratio of liquid to raw material, extraction time, extraction temperature and extraction times were 40, 2h, 100℃ and 2, respectively. The size-exclusion column chromatography was used to purify crude polysaccharides and determine their molecular weights .The results showed the rape polysaccharides were composed of two pure polysaccharides, which molecular weights were 63.51kD and 49.58kD, respectively. |
| |
| Keywords: | flower of rape. polysaccharide extraction purification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
|
