In situ detection of tandem DNA repeat length |
| |
Authors: | R Yaar P Szafranski CR Cantor CL Smith |
| |
Affiliation: | Center for Advanced Biotechnology, Boston University, MA 02215, USA. |
| |
Abstract: | A simple method for scoring short tandem DNA repeats is presented. An oligonucleotide target, containing tandem repeats embedded in a unique sequence, was hybridized to a set of complementary probes, containing tandem repeats known lengths. Single-stranded loops structures formed on duplexes containing a mismatched (different) number of tandem repeats. No loop structure formed on duplexes containing a matched (identical) number of tandem repeats. The matched and mismatched loop structures were enzymatically distinguished and differentially labeled by treatment with S1 nuclease and the Klenow fragment of DNA polymerase. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|