Fluorescent staining with bromocresol purple: A rapid method for determining yeast cell dead count developed as an assay of killer toxin activity |
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Authors: | Helena Kurzweilov ,Karel Sigler |
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Affiliation: | Helena Kurzweilová,Karel Sigler |
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Abstract: | A method is described for detecting yeast cells with plasma membrane damage, based on cell staining with bromocresol purple (BCP) which has a convenient fluorescence after entering the cells at pH below 5·2. The method was used to determine the activity of Saccharomyces cerevisiae pore-forming killer toxin K1 in commonly used lethal units. The BCP test is rapid and as precise as the plating test. |
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Keywords: | Bromocresol purple killer toxin Saccharomyces cerevisiae fluorescence staining yeast |
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