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Low-temperature scanning electron microscopy in fungus-nematode interaction
Authors:E. Den Belder  A. Boekestein  J. W. J. Van Esch  F. Thiel
Abstract:A comparative study has been made of different preparation techniques used for the scanning electron microscope (SEM), with regard to their application to fungus-nematode interaction. The preparation of frozen-hydrated specimens of both healthy and Arthrobotrys-oli-gospora-infected second-stage larvae of the root-knot nematode (Meloidogyne sp.) is described, and the results are compared with those obtained by critical point-drying and freeze-drying. In all cases the frozen-hydrated specimens consistently showed the best preservation.
Keywords:Arthrobotrys-oligospora  nematode-capturing fungi  root-knot nematode  meloidogyne  low temperature scanning electron microscopy  freeze substitution  critical-point drying
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