The DNA sequence analysis of the HAP4–LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast |
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Authors: | Genevive Chret Chantal Pallier Michle Valens Bertrand Daignan-Fornier Hiroshi Fukuhara Monique Bolotin-Fukuhara Frdric Sor |
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Affiliation: | Geneviève Chéret,Chantal Pallier,Michèle Valens,Bertrand Daignan-Fornier,Hiroshi Fukuhara,Monique Bolotin-Fukuhara,Frédéric Sor |
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Abstract: | The nucleotide sequence of a 19 000 base pair region from the left arm of chromosome XI of Saccharomyces cerevisiae has been determined and analysed. It covers the HAP4–GFA1–LAP4 loci already described. As expected HAP4, GFA1 and LAP4 genes have been found and six new open reading frames (ORFs) with a coding capacity of more than 100 amino acid residues have been identified. One of them (YKL461) shows a high degree of identity with an aspartate aminotransferase gene. This raises the question of a second aspartate aminotransferase gene in yeast. A second ORF (YKL462) shows features compatible with a membranous localization. The other ORFs do not show a similarity with any known gene. A member of the highly repetitive ‘CAT’ DNA sequence is present. |
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Keywords: | Saccharomyces cerevisiae yeast chromosome XI HAP4 GFA1 LAP4 AAT1 aspartate aminotransferase |
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