Enrichment of docosahexaenoic acid from tuna oil via lipase-mediated esterification under pressurized carbon dioxide |
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| Affiliation: | 1. Department of Food & Nutrition, Korea University, Jeongneung-dong, Seongbuk-Gu, Seoul 136-703, Republic of Korea;2. Korea Food Research Institute, Seongnam-si, Gyunggi-do 463-746, Republic of Korea;3. Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-749, Republic of Korea;4. Department of Public Health Sciences, Graduate School, Korea University, Seoul 136-703, Republic of Korea;1. Enzyme and Fermentation Technology Laboratory, College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, PR China;2. State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, PR China;1. CNRS, Univ. Bordeaux, ICMCB, UPR 9048, F-33600 Pessac, France;2. Center for Materials Crystallography, Department of Chemistry and iNANO, Aarhus University, Aarhus, Denmark;1. Biotechnology, Technological University of Morelia, 58200 Morelia, Mich., Mexico;2. Department of Biotechnology and Food Science, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain;1. Department of Biochemistry, Kurukshetra University, Kurukshetra 136119, India;2. Indian Institute of Integrative Medicine, Jammu 180001, India |
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| Abstract: | This study focused on the use of pressurized CO2 as a reaction medium for the enrichment of docosahexaenoic acid (DHA) from tuna oil fatty acids via lipase-mediated esterification. Of the three lipases tested, Lipozyme RM IM from Rhizomucor miehei was selected for further study. Enzyme loading, water addition, and reaction time were also explored. Near-supercritical CO2, prepared at 25 °C and 8.3 MPa, was the most effective reagent tested for enriching DHA from the residual fatty acid fraction. In addition to near-supercritical CO2, optimal conditions included addition of 0.2 wt% (based on total substrates) water, enzyme loading of 5 wt% (based on total substrates), and a reaction time of 18 h. The DHA concentration and recovery yield for the residual fatty acid fraction under these optimal conditions were 75.8 wt% and 81 wt%, respectively. |
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| Keywords: | Docosahexaenoic acid (DHA) Ethanol Near-supercritical carbon dioxide Tuna oil fatty acids |
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