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响应面法优化薄荷叶总黄酮提取工艺及抗氧化活性
引用本文:侯学敏,李林霞,张直峰,闫桂琴.响应面法优化薄荷叶总黄酮提取工艺及抗氧化活性[J].食品科学,2013,34(6):124-128.
作者姓名:侯学敏  李林霞  张直峰  闫桂琴
作者单位:山西师范大学生命科学学院,生物多样性研究所,山西 临汾 041004
摘    要:目的:确定薄荷叶中总黄酮超声辅助提取最佳工艺条件,并对其抗氧化活性进行评价。方法:在单因素试验基础上,以总黄酮的提取率为指标,响应面法优化其超声提取条件;并通过其总黄酮对羟自由基和超氧阴离子自由基的清除作用来研究其抗氧化活性。结果:薄荷叶总黄酮的最佳提取条件:乙醇体积分数80%、液料比40:1、提取时间118min、提取温度62℃,此条件下,薄荷叶总黄酮得率达6.11%。薄荷叶总黄酮对羟自由基和超氧阴离子自由基有较强的清除作用,并与总黄酮质量浓度呈一定的正相关关系。结论:利用响应面法分析结果可靠,得到了薄荷叶总黄酮超声辅助提取的最佳工艺条件,且实验结果还表明薄荷叶具有较强的抗氧化活性。

关 键 词:薄荷  总黄酮  超声提取  响应面  抗氧化  
收稿时间:2012-05-27

Total Flavonoids from Mentha haplocalyx Briq. Leaves: Optimization of Extraction Process by Response Surface Methodology and Antioxidant Activity
HOU Xue-min,LI Lin-xia,ZHANG Zhi-feng,YAN Gui-qin.Total Flavonoids from Mentha haplocalyx Briq. Leaves: Optimization of Extraction Process by Response Surface Methodology and Antioxidant Activity[J].Food Science,2013,34(6):124-128.
Authors:HOU Xue-min  LI Lin-xia  ZHANG Zhi-feng  YAN Gui-qin
Affiliation:School of Life Science, Institute of Biodiversity, Shanxi Normal University, Linfen 041004, China
Abstract:Objective: To explore the optimal ultrasonic-assisted extraction conditions of total flavonoids from Mentha haplocalyx Briq. leaves and evaluate the antioxidant activity. Methods: Based on the single-factor tests, the ultrasonic extraction conditions of total flavonoids from Mentha haplocalyx Briq. leaves were optimized by response surface methodology in order to increase the extraction rate of total flavonoids. Meanwhile, the antioxidant activity of total flavonoids was assessed by scavenging assays of hydroxyl and superoxide anion free radicals. Results: The optimal extraction conditions of total flavonoids were ethanol concentration of 80%, material-liquid ratio of 1:40, extraction time of 118 min and extraction temperature of 62 ℃. Under the optimal conditions, the maximum extraction rate of total flavonoids was 6.11%. Extracted total flavonoids had strong scavenging capacity on hydroxyl and superoxide anion free radicals in a dose-dependent manner. Conclusion: Response surface methodology is applicable for the optimization of ultrasonic extraction of total flavonoids from Mentha haplocalyx Briq. leaves. The total flavonoids from Mentha haplocalyx Briq. leaves have strong antioxidant capacity. These investigations will be theoretical reference for further development and utilization of Mentha haplocalyx Briq..
Keywords:Mentha haplocalyx Briq    flavonoids  ultrasonic extraction  response surface methodology  antioxidant activity  
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