| 高效液相色谱法测定黑果枸杞果实中花色苷的含量 |
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| 引用本文： | 林丽,李进,丁成丽.高效液相色谱法测定黑果枸杞果实中花色苷的含量[J].食品科学,2013,34(6):164-166. |
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| 作者姓名： | 林丽 李进 丁成丽 |
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| 作者单位： | 1.新疆师范大学生命科学学院，新疆 乌鲁木齐 830054；2.中国科学院新疆理化技术研究所分析测试中心，新疆 乌鲁木齐 830054 |
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| 基金项目： | 国家自然科学基金面上项目(30960047);新疆科技支疆项目(200991240);新疆师范大学研究生科技创新项目(20111213) |
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| 摘 要： | 目的：建立利用高效液相色谱测定黑果枸杞果实中花色苷含量的方法。方法：采用Kromasil C18色谱柱(4.6mm×250mm，5μm)为固定相，0.2%甲酸溶液-乙腈(98:2)混合溶液为流动相梯度洗脱，用紫外检测器于520nm波长处检测，控制流量为1mL/min。结果：在43～935μg/mL范围内，相关系数为0.9994，RSD为2.73%，方法加标回收率为101.01%，相对标准偏差(n=6)为2.12%。结论：本方法简便，结果可靠，适用于黑果枸杞花色苷含量的测定。
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| 关 键 词： | 高效液相色谱 花色苷 黑果枸杞 |
| 收稿时间： | 2011-12-14 |
Determination of Anthocyanins in Fruits of Lycium ruthenicum Murr. by HPLC |
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| LIN Li,LI Jin,DING Cheng-li.Determination of Anthocyanins in Fruits of Lycium ruthenicum Murr. by HPLC[J].Food Science,2013,34(6):164-166. |
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| Authors: | LIN Li LI Jin DING Cheng-li |
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| Affiliation: | 1. Life Sciences Institute, Xinjiang Normal University, ürümqi 830054, China；2. Center of Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, ürümqi 830054, China |
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| Abstract: | Objective: A HPLC method for the determination of anthocyanins in fruits of Lycium ruthenicum Murr. was developed. Methods: A Kromasil C18 (4.6 mm × 250 mm, 5 μm) column was used as the chromatographic column and a mixture of 0.2% HCOOH-H2O and acetonitrile (98:2) was used as the mobile phase at a flow rate of 1 mL/min for gradient elution. UV detection wavelength was set up at 520 nm. MAL standards were used for calibration. Results: An excellent linear relationship was achieved in the range of 43–935 μg/mL with correlation coefficient of 0.9994 and RSD of 2.73%. The recovery rate of in spiked samples was 101.01% with RSD (n = 6) of 2.12%. Conclusion: This developed method is simple, stable, fast, and reproducible and without the interference of impurities, so that it can be used for determining the content of anthocyanins in fruits of Lycium ruthenicum Murr.. |
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| Keywords: | HPLC anthocyanins Lycium ruthenicum Murr |
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