沙枣多糖EAP-2的提取纯化及初步分析

沙枣果肉干粉经脱脂,热水提取,醇析,三氯乙酸结合四次Sevag法脱蛋白,活性炭脱色得到沙枣多糖(EAP)粗品,再经DEAE-纤维素柱层析、透析得到纯化的3个级分EAP-1、EAP-2和EAP-3。对糖含量较高的精多糖EAP-2进行定性化学反应和紫外光谱鉴定表明,EAP-2不含蛋白质、核酸和淀粉,D-半乳糖醛酸的含量为2.3%。经高效液相色谱对EPA-2的单糖基分析表明,EAP-2中含有L-鼠李糖、D-木糖、L-阿拉伯糖、D-果糖、D-甘露糖、D-葡萄糖及D-半乳糖7种中性糖。红外光谱鉴定表明,EPA-2具有多糖类物质的结构特征。

Extraction, Purification and Preliminary Analysis of Eleagnus angustifolius L. Polysaccharide-2

The crude Eleagnus angustifolius L.polysaccharide was obtained by a series of steps of degreasing, hot water extraction, alcohol precipitation, trichloroacetic acid treatment and further purified four times Sevag deproteinization and decoloring. EAP-1, EAP-2 and EAP-3 were gained by DEAE-cellulose and dialysis method. Qualitative chemical reaction and UV analysis indicated that EAP-2 is an acid polysaccharide with 2.3% D-galacturonic acid and without any protein, nuclear acid and starch. The units of the neutral monosaccharide are identified by HPLC as rhanmose, xylose, arabinose, fructose, mannose, glucose and galactose. The polysaccharide structure of EAP-2 is identified by IR.