Application of conducting poly(aniline‐co‐pyrrole) film to cholesterol biosensor |
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| Authors: | Pratima R Solanki Suman Singh Nirmal Prabhakar M K Pandey B D Malhotra |
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| Affiliation: | 1. Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi‐110012, India;2. Email:bansi.malhotra@gmail. com |
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| Abstract: | Cholesterol oxidase (ChOx) has been covalently immobilized onto poly(aniline‐co‐pyrrole), electrochemically deposited onto indium‐tin‐oxide (ITO) glass plates, using glutaraldehyde as a crosslinker. These poly (An‐co‐Py)/ChOx films have been characterized using UV–visible spectroscopy fourier transform infrared spectroscopy, scanning electron microscopy, and photometric and amperometric techniques, respectively. The poly(An‐co‐Py)/ChOx bioelectrodes have been utilized for cholesterol estimation in the range of 1–10 mM. The ChOx activity in poly(An‐co‐Py)/ChOx bioelectrode has been found to be the highest at pH 7.0 at 25°C. The sensitivity and stability of poly(An‐co‐Py)/ChOx bioelectrode have been experimentally determined as 93.35 μA/mM and 10 weeks at 4°C, respectively. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2007 |
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| Keywords: | polyaniline polypyrrole copolymer cholesterol cholesterol oxidase biosensor electrochemical polymerization |
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