普通烟草碳酸酐酶家族基因的生物信息学分析

为挖掘普通烟草碳酸酐酶基因的信息并探讨其功能，本研究利用生物信息学方法，在烟草基因组数据库中对普通烟草碳酸酐酶家族成员进行了检索，并对其理化性质、遗传进化、基因结构、蛋白保守基序、顺式作用元件和组织表达模式进行了分析。结果显示，在普通烟草中至少含有9个α和6个β亚家族成员。在进化关系上，不同亚家族成员之间，序列同源性较低；与水稻相比，拟南芥和普通烟草两个亚家族间的亲缘关系较近。亚细胞定位分析结果显示，烟草α亚家族成员在细胞壁、细胞膜、线粒体、叶绿体、细胞质等细胞器中均有分布，而β亚家族成员均存在于叶绿体中。基因结构和保守基序分析说明，各亚家族成员的基因结构和蛋白保守基序呈现较高的一致性。启动子顺式作用元件分析显示，烟草碳酸酐酶基因的启动子中，均含有多个参与光反应、激素响应、非生物胁迫和机械损伤等相关的顺式作用元件。基因组织表达模式分析表明，烟草的碳酸酐酶基因不同成员在不同组织表达水平存在差异，并呈现出时空特异性。本研究结果为烟草碳酸酐酶基因的功能研究奠定了基础。

Bioinformatics Analysis of Carbonic Anhydrase Family Genes in Tobacco

In order to find out the information of carbonic cnhydrase (CA) genes in tobacco and explore their functions, the members of common tobacco CA family were identified from the tobacco genome database, and the physical and chemical properties, genetic evolution, gene structure, conserved motifs of proteins, cis-acting elements and tissue expression patterns were analyzed. The results showed that there were at least 9 alpha and 6 beta subfamily members in tobacco. On evolutionary relations, lower sequence homology was observed between different subfamily members. Compared with rice, CA members between Arabidopsis thaliana and common tobacco are more closely related. Subcellular localization analysis showed that tobacco alpha subfamily members were distributed in cell wall, cell membrane, mitochondria, chloroplast, cytoplasm and other organelles, while beta subfamily members were present in chloroplast. The analysis of gene structure and conserved motifs showed that the gene structure and conserved motifs of proteins of each subfamily were highly consistent. Analysis of the cis-acting elements of the promoter showed that the promoter of the carbonic anhydriase genes in tobacco contains several cis-acting elements involved in light reaction, hormone response, abiotic stress and mechanical damage. The expression pattern analysis confirmed that the expression levels of carbonic anhydrase genes in different subfamilies of tobacco were different in different tissues and showed the specificity of time and space. The results of this study laid a foundation for the functional study of tobacco carbonic anhydrase genes.