The pectin substance used to detect the activity of pectinase suitable for bioscouring |
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| Authors: | Kang-li Fu Jing-ping Liu |
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| Affiliation: | 1. Key Laboratory of Science &2. Technology of Eco-Textiles, College of Chemistry, Chemical Engineering &3. Biotechnology, Donghua University, Ministry of Education, Shanghai, China. |
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| Abstract: | Structural characteristics of pectins from cotton fabrics, ramie, and apple were investigated by titration method and ATR–FTIR spectra. Pectin from cotton fabrics and ramie showed similar structure characteristics, both these two pectins showed big structural difference with pectin from apple. The activities of five pectinases were determined by using these three pectins as substrates. These five pectinases showed similar hydrolysis action on pectin from ramie and cotton fabrics, they also showed a great difference in the hydrolysis of pectin from apple and cotton fabrics. Moreover, bioscouring effect with these five pectinases was investigated, the pectinases with stronger hydrolysis action on ramie pectin displayed better bioscouring effect and the pectinase suitable for bioscouring was selected out. The bioscouring effect of the pectinase selected out was studied and showed almost perfect bioscouring effect. |
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| Keywords: | pectin-containing substrates pectinase enzyme activity bioscouring |
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