重组聚合酶扩增技术在沙门氏菌检测中的应用

沙门氏菌是重要的致病菌,能引起人和动物肠道疾病,因此检测沙门氏菌具有十分重要的卫生学意义。重组酶聚合酶扩增技术(recombinase polymerase amplification,RPA)是一种新型的恒温核酸扩增技术,具有反应快速、灵敏度高、特异性强等优点。利用RPA技术检测沙门氏菌,具有较大的应用前景和市场。本文对近年来应用RPA技术检测沙门氏菌的研究进行综述,以期为相关进一步研究及应用提供参考。

Application of recombinant polymerase amplification in salmonella detection

Salmonella is an important pathogen that can cause intestinal diseases in human and animal, so that Salmonella has very important hygiene significance. Recombinase polymerase amplification (RPA) technology is a new type of constant temperature nucleic acid amplification technology, which has the advantages include fast reaction, high sensitivity and strong specificity. Using RPA technology to detect Salmonella has a great application prospect and market. This article reviews recent studies on the application of RPA technology to detect Salmonella in order to provide references for further research and applications.