响应面法超声波提取胡芦巴薯蓣皂苷元的研究

采用超声波法对胡芦巴中薯蓣皂苷元的提取工艺过程进行了研究。在单因素实验基础上根据中心组合实验设计原理,采用四因素五水平的响应面分析法对提取工艺进行了优化。通过响应面实验及回归分析得出最佳工艺条件为:原料粒径50目,料水质量比1∶16.2,浸泡7 h后,在频率20 kHz、功率200 W超声强度下提取35 min,胡芦巴中薯蓣皂苷元的提取率可达87.65%。验证实验表明,所得二次模型方程能较好地预测实验结果。

Extraction of diosgenin from fenugreek with ultrasound technology optimized by response surface methodology

The optimization of extraction process of diosgenin from fenugreek with ultrasound technology was investigated.Experimental factors and levels were firstly selected by one-factor test.According to the central composite experimental design principle,the response surface methodology with 4 factors and 5 levels was adopted.The factors influencing the technological parameters were determined by means of regression analysis.The results show that the optimum conditions of diosgenin extraction are as follows: the grain size of raw material is 50 mesh,the ratio of water to material is 1∶16.2,steeping time is 7 h,frequency of ultrasound is 20 kHz,the power is 200 W and ultrasound extraction time is 35 min,and the extraction rate can be up to 87.65%.The validation test shows that the prediction values of the model are well agreed with the experimental values.