99Tcm硫化铼胶体用于前哨淋巴结检测的生物实验——注射剂量与明胶浓度的影响

采用NANOCIS药盒制备了⁹⁹Tc^m-硫化铼胶体。对标记溶液进行10、100、500及715倍稀释，将原液及稀释液由小鼠脚掌皮内注射给药，于给药后1、4 h处死小鼠，取腘窝淋巴结（SLN）、腰淋巴结（次级淋巴结，2LN）、注射点（Injection Site）、肝、脾、血，测量放射性计数，计算放射性摄取率，研究注射剂量及明胶浓度对⁹⁹Tc^m-硫化铼胶体在小鼠前哨淋巴结（SLN）的放射性摄取及体内分布的影响。结果表明，随着注射剂量的降低，前哨淋巴结的放射性摄取相应减少，而注射点的摄取和前哨淋巴结提取率（在此指腘窝淋巴结提取率，PE%）相应增加；注射体积增加时，在注射点的放射性浓集增加，而前哨淋巴结提取率降低；当稀释液中明胶的浓度由0.03 g/L增加至3 g/L时，给药后可见前哨淋巴结的放射性摄取增加，而注射点的放射性摄取和PE%则有所降低。以上结果提示，NANOCIS药盒中明胶发挥了稳定剂的作用，而注射剂量和注射体积是影响该类药物淋巴结摄取及体内分布的关键因素。

Biodistribution of 99Tcm Labelled Colloidal Rhenium Sulphide for Sentinel Node Detection:the Effects of Dosage and Concentration of Gelatin

99Tcm labelled colloidal rhenium suulphide was prepared from NANOCIS○R kit which was currently used for sentinel node detection clinically.After the labelling,the reaction solution was diluted into 10,100,500 and 715 folds,and respectively injected to the animals and biodistribution studies were carried out subsequently 1 h and 4 h postinjection.The effect of injected volume was also examined by administration of the same quantity but different volume of the labelled agent.In a separate biodistribution study,3 g/L gelatin solution was used for the dilution and the function of gelatin was further evaluated.The results of biodistribution demonstrated that the uptake of SLN decreased while the retention at the injection site as well as popliteal extraction rate(PE%,refers to sentinel node extraction rate in this case) increased as the injected dosage went down.If more volume was injected as the quantity remained the same,the retention at the injection site increased and PE% decreased.When extra gelatin was added,the retention at the injection site and PE% decreased as the uptake of SLN increased.It could be concluded that gelatin in the NANOCIS kit acts as a stabilizer that will prevent the formation of aggregates and the dosage administered to the animals especially after dilution of reaction solution played a crucial role in the biological evaluation on SLN imaging agents.