Deconjugation of bile salts by Lactobacillus acidophilus isolates |
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| Affiliation: | 1. Laboratory of Milk Science & Microbiology, School of Agricultural Biotechnology, Seoul National University, Suwon 441-744, South Korea;2. Department of Food Science & Agricultural Chemistry, McGill University, Ste-Anne-de-Bellevue, Quebec, Canada H9X 3V9;3. Research & Development Center, Korea Yakult Co., Ltd., Yongin 449-900, South Korea;1. National Institute of Food Science and Technology, University of Agriculture, Faisalabad, 38000, Pakistan;2. School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China;3. Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, China;1. School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China;2. Dalian Probiotics Function Research Key Laboratory, Dalian Polytechnic University, Dalian 116034, China;3. Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China |
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| Abstract: | Deconjugation of bile salts by Lactobacillus acidophilus strains isolated from men, fermented milk, and pigs resulted in precipitate halo, opaque granular white colonies, shiny precipitate halo or clear zone around the microbial colonies on bile salt–MRS agar plates depending on the type of bile salts added. None of the L. acidophilus cultures tested exhibited 7α-dehydroxylase activity that transforms cholate into deoxycholate. L. acidophilus SNUL020 and SNUL01 deconjugated both taurocholate (TCA) and glycocholate (GCA) at similar rates, while L. acidophilus FM01 deconjugated GCA more rapidly than TCA. Most L. acidophilus strains tested precipitated more soluble cholesterol in the media containing taurochenodeoxycholate (TCDCA) and taurodeoxycholate (TDCA) than with TCA and GCA. In the medium containing TDCA, L. acidophilus SNUL020, SNUL01, and FM01 precipitated more than 50% of soluble cholesterol. All L. acidophilus strains tested were more resistant to taurine- than to glycine-conjugated bile salts. Fecal excretions of cholesterol and deoxycholate increased slightly with acidophilus milk intake containing live cells of L. acidophilus SNUL01. |
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