Synthesis of an affinity adsorbent based on silica gel and its application in endotoxin removal |
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| Affiliation: | 1. Key Laboratory of Molecular Engineering of Polymers, Ministry of Education, Department of Macromolecular Science, Fudan University, Shanghai 200433, PR China;2. Zhongshan Hospital, Fudan University, Shanghai 200032, PR China;3. Institute of Biochemistry, Biocenter, Goethe University, Max-von-Laue-Strasse 9, 60438 Frankfurt/M.;4. Lehrstuhl für Theoretische Chemie, Ruhr-University Bochum, 44780 Bochum;5. Cluster of Excellence–Macromolecular Complexes, Goethe University, Max-von-Laue-Str. 9, 60438 Frankfurt/M., Germany;1. Applied Mathematics & Computational Science, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia;2. Mechanical Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia;3. Center for Numerical Porous Media (NumPor), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia;4. Earth Science & Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia |
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| Abstract: | An affinity media for endotoxin removal based on silica gel was prepared by activation with silane coupling agent and subsequent conjugation with histidine (His) as a ligand. The influence of pore size and particle dimension of the silica gel and the grafting conditions such as reaction temperature and concentration of His in solution on the grafting yield of His were studied by means of IR, thermogravimetric analysis and elemental analysis. The results show that the silica gel with a particle size of 200 μm and a pore size of about 12 nm was a good carrier material for the preparation of the affinity adsorbent. Under the optimal conditions, histidine was covalently immobilized on silica gel at about 20 mg/g, with a corresponding maximum endotoxin adsorption capacity qm in contaminated water of about 1.2 mg/g and an apparent dissociation constant Kd of about 1350 μg/L. The adsorbent had little nonspecific adsorption from a protein-containing solution. |
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