Immobilization of mushroom polyphenol oxidase on poly(allyl glycidyl ether-co-ethylene glycol dimethacrylate) macroporous beaded copolymers |
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| Affiliation: | 1. Biochemical Engineering Department, Chemical Engineering & Process Development Division, National Chemical Laboratory, Pune 411008, India;2. Polymer Science and Engineering Division, National Chemical Laboratory, Pune 411008, India;1. School of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, PR China;2. Department of Chemical Engineering, Kunming Metallurgy School, Kunming 650031, PR China;3. School of Life Science, Yunnan Normal University, Kunming 650500, PR China;1. Institute for Advanced Studies in Basic Sciences (IASBS), 45137-66731 Zanjan, Iran;2. Department of Chemistry, College of Sciences, Shiraz University, Shiraz 71454, Iran;3. Nicolaus Copernicus University, Department of Chemistry, 87-100 Torun, Poland;4. Institute of Mathematics, Physics and Mechanics & Faculty of Civil and Geodetic Engineering, University of Ljubljana, Jadranska 19, SI-1000 Ljubljana, Slovenia;1. School of Pharmaceutical Science, Shandong University, Jinan 250012, China;2. Xintai Institute of Skin Diseases, Xintai 271200, China;3. The Second Hospital of Shandong University, Jinan 250033, China;4. Faculty of Pharmaceutical Sciences, School of Health Sciences, University of Iceland, Iceland;1. Departament d’Enginyeria Química, Universitat Politècnica de Catalunya, ETSEIB, Diagonal 647, Barcelona 08028, Spain;2. Health and Environmental Microbiology Lab, UNESCO Chair on Sustainability, Universitat Politècnica de Catalunya, ESEIAAT, Edifici Gaia, Pg. Ernest Lluch/Rambla Sant Nebridi, Terrassa 08222, Spain;1. Department of Chemical Engineering, Norwegian University of Science and Technology, 7491 Trondheim, Norway;2. Department of Chemical and Biological Engineering, UCB 424, University of Colorado, Boulder, CO 80309, USA |
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| Abstract: | Functional, macroporous, beaded copolymers containing epoxy groups were synthesized for immobilization of polyphenol oxidase (PPO) from edible mushroom (Agaricus bisporus). The effect of incorporation of two different sets of monomers such as glycidyl methacrylate (GMA) and allyl glycidyl ether (AGE) and the effect of cross-linking agent ethylene glycol dimethacrylate (EGDM) with varying cross-link densities on binding and expression of mushroom PPO activity were studied. The effect of porogen viz. cyclohexanol and hexanol on PPO immobilization was studied. AGE copolymers with hexanol as a porogen were found to give higher binding and expression of PPO activity than GE polymers. Cross-linking of amino groups of enzyme with 5% glutaraldehyde for 6 h gave a stable binding of PPO on AGE-75(Hex) polymer with storage half-life of approximately 25 days. Under optimum conditions, AGE-75(Hex) polymer gave 70.3% of activity yield while percent retention of PPO activity was found to be 83.5%. Immobilized PPO showed a broader pH, higher temperature and excellent storage stability. |
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