Adsorption of bovine serum albumin to a polymer brush prepared by atom-transfer radical polymerization in a porous inorganic membrane |
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| Authors: | Hidetaka Kawakita Hiroyasu Masunaga Kanako Nomura Kazuya Uezu Isamu Akiba Satoshi Tsuneda |
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| Affiliation: | (1) Advanced Research Institute for Science & Engineering, Waseda University, Kyushu, Kitakyushu 808-0135, Japan;(2) Present address: Department of Applied Chemistry, Saga University, Saga 840-8502, Japan;(3) Kitakyushu Foundation for the Advancement of Industry Science and Technology, Kitakyushu 808-0135, Japan;(4) Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu, Kitakyushu 808-0135, Japan;(5) Department of Chemical Engineering, Waseda University, 169-8555 Kitakyushu, Japan |
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| Abstract: | Protein adsorption was performed by a polymer brush prepared by atom-transfer radical polymerization (ATRP) to a porous inorganic
membrane. The porous inorganic membrane, Shirasu Porous Glass made from silica, was modified with a halogen-containing compound
to bind the active species for the polymerization. Glycidyl methacrylate was polymerized from the halogen compound by ATRP
for a prescribed time, and subsequently chemically modified. The progression of the chemical modification allowed the membrane
to lower the phosphate-buffer flux of the porous membrane due to the attachment of the polymer brush. Bovine serum albumin
(BSA), as a model protein, was adsorbed at 12 mg per gram of the membrane in permeating BSA solution through the polymer-brush-attached
porous membrane. |
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| Keywords: | Shirasu Porous Glass Atom-transfer radical polymerization Bovine serum albumin Polymer brush Adsorption |
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