| 酸解-酶膜联用法检测谷氨酰胺 |
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| 引用本文： | 陈奎发,蒋楠,杨艳,焦鹏,曹竹安.酸解-酶膜联用法检测谷氨酰胺[J].食品与发酵工业,2002,28(9):26-28. |
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| 作者姓名： | 陈奎发 蒋楠 杨艳 焦鹏 曹竹安 |
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| 作者单位： | 清华大学化学工程系,北京,100084 |
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| 摘 要： | 研究了谷氨酰胺在酸性条件下的水解特性后 ,建立了酸解 酶膜联用的谷氨酰胺定量分析方法。此方法的线性范围为 10～ 40mmol/L ,相关系数R =0 .999,分析误差为 0 .1mmol/L ,测量所需时间约为 30min。
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| 关 键 词： | 谷氨酰胺 谷氨酸 定量分析 生物传感器 |
| 修稿时间： | 2002年4月3日 |
Determination of Glutamine with the Method of Acidic Hydrolysis Coupled with Enzyme Membrane |
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| Chen Kuifa,Jiang Nan,Yang Yan,Jiao Peng,Cao Zhu''''an.Determination of Glutamine with the Method of Acidic Hydrolysis Coupled with Enzyme Membrane[J].Food and Fermentation Industries,2002,28(9):26-28. |
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| Authors: | Chen Kuifa Jiang Nan Yang Yan Jiao Peng Cao Zhu'an |
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| Abstract: | The properties of glutamine hydrolysis in the acidic solution were investigated and the analytical method of acidic hydrolysis coupled with enzyme membrane for glutamine was established. The relation was linear from 10 to 40?mmol/L(R=0.999) and the error was within 0.1?mmol/L. The process can be finished in 30?min. |
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| Keywords: | glutamine glutamate quantitative analysis biosensor |
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