酶解脱脂蚕蛹蛋白制备ACE抑制肽

以实验室自制的脱脂蚕蛹蛋白为原料，利用酶工程技术，通过对中性蛋白酶、碱性蛋白酶、木瓜蛋白酶、复合蛋白酶、风味蛋白酶、胰蛋白酶等的筛选及单因素和响应面优化试验，对ACE抑制肽的制备工艺条件进行较系统的研究。结果表明：选择碱性蛋白酶作为脱脂蚕蛹蛋白制备ACE抑制肽的酶，制备ACE抑制肽的最佳工艺条件为料液比11.88:100、温度50.22℃、pH 9.46、加酶量7.03%、酶解4h。在此条件下制备的ACE抑制肽的ACE抑制率达到41.98%。

Enzymatic Preparation of ACE-Inhibitory Peptide from Pupa Protein

Defatted pupa protein was prepared in our laboratory and used further to prepare ACE-inhibitory peptide by enzymatic hydrolysis.The best protease for preparing ACE-inhibitory peptide from pupa protein was chosen out of neutral protease,alcalase,papain,protamex,flavourzyme and trypsin.The hydrolysis process was optimized using response surface methodology.Neutral protease was found to be the best among all investigated proteases.The optimal hydrolysis conditions were determined as follows: substrate concentration of 11.88:100,hydrolysis temperature of 50.22 ℃,hydrolysis pH of 9.46,and enzyme concentration of 7.03%,hydrolysis time of 4 h.Under these conditions,pupa protein hydrolysate with an ACE-inhibitory rate of 41.98% was obtained.