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高产S-腺苷甲硫氨酸酵母菌株的筛选
引用本文:刘勇,刘建玲,王璋. 高产S-腺苷甲硫氨酸酵母菌株的筛选[J]. 食品与发酵工业, 2008, 34(12)
作者姓名:刘勇  刘建玲  王璋
作者单位:1. 西北大学生命科学学院,陕西,西安,710069;中国食品发酵工业研究院,北京,100027
2. 西北大学生命科学学院,陕西,西安,710069
3. 中国食品发酵工业研究院,北京,100027
摘    要:从土壤和酒曲中对产S-腺苷甲硫氨酸的酵母菌株进行了筛选,通过HPLC外标法测定得到8株产量较高的酵母菌株。对高氯酸细胞破壁和酵母细胞水解作用条件进行优化,确定了高氯酸提取S-腺苷甲硫氨酸的理想条件为2.5mol/L高氯酸10mL,时间为5h,菌体量为0.6g。

关 键 词:土壤  酒曲  S-腺苷甲硫氨酸  高氯酸  优化

Isolation of Yeast Strains for S-adenosyl-L-methionine Production
Liu Yong,Liu Jianling,Wang Zhang. Isolation of Yeast Strains for S-adenosyl-L-methionine Production[J]. Food and Fermentation Industries, 2008, 34(12)
Authors:Liu Yong  Liu Jianling  Wang Zhang
Abstract:The yeast strains producing S-adenosyl-L-methionine(SAM)were isolated from the soil and solid koji samples.Eight yeast stains of higher SAM productivities were acquired with an external standard analytical method of HPLC.The best conditions of extracting SAM form the yeast cells were 2.5mol/l perchloric acid 10ml,5h hydrolytic time and 0.6g biomass by optimizing the condition between perchlorate and cells of yeast.
Keywords:S-adenosyl-L-methionine  screening  soil  solid koji
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