不同豆浆饮品对普通及高脂饲料饲喂小鼠健康的影响

研究普通日粮和高脂饲料条件下不同豆浆饮品对小鼠健康的影响。采用4种不同豆浆饮品(自磨非转基因豆浆F、自磨转基因豆浆G、两种常见市售豆浆K和M)灌胃C57小鼠,并分别饲喂普通日粮和高脂饲料,10周后处死,测定其体重增长率、肝脏系数和结肠长度等参数,并用生化分析仪测定小鼠血清葡萄糖(Glucose,GLU)、甘油三脂(Triglycerides,TG)、总胆固醇(total cholesterol,CHOL)、高密度脂蛋白胆固醇(High density lipoprotein cholesterol,HDL-C)和低密度脂蛋白胆固醇(Low density lioprotein cholesterol,LDL-C)等指标。用不同豆浆处理人结肠癌细胞HCT-116,检测其对细胞增殖和脂滴积累的影响。结果显示,所有豆浆都不同程度地降低了小鼠血液TG,且市售豆浆M还降低了小鼠血液GLU和CHOL浓度;豆浆处理后结肠癌细胞增殖活性得到明显抑制,但是对油酸诱导的细胞增殖和脂滴积累影响不显著。不同豆浆由于营养及功能活性物质的含量不同,对小鼠血糖、血脂和胆固醇等参数的影响不同,市售豆浆相对自磨豆浆更利于减轻高脂饲料带来的健康副作用。

Effects of different soybean milk on the health of mice fed with normal and high-fat diets

The effects of different kinds of soybean milk on mice under normal diet and high fat feed conditions were researched. Four different kinds of soybean milk, including non-transgenic home-made soybean milk F, transgenic home-made soybean milk G and two kinds of common commercial ready-made soybean milk K and M, were selected to feed the C57 mice by gavage. Normal and high-fat diets were added, respectively. The executions were performed after 10 weeks. The weight growth rate, liver coefficient and colon length of the mice were measured. The concentrations of GLU, TG, CHOL, HDL-C and LDL-C in mice serum were determined by a biochemical analyzer. Human colon cancer cell line HCT-116 was used to investigate the influences of different soybean milks on the proliferation of cells and accumulation of lipid droplets. Results showed that the level of blood TG in mice was reduced by all of the soybean milks. Commercial ready-made soybean milk M could lower the concentrations of GLU and CHOL. The cell proliferation were significantly decreased by the soybean milks, however, the decrease were non-significant when the proliferation were induced by the oleic acid. Same results were found in the accumulations of lipid droplets. Different soybean milks cocld bring different effects on concentrations of GLU, TG, and CHOL etc in mice serum for their various contents of nutritional and functional factors. The commercial ready-made soybean milk was more conducive to reduce the adverse impact brought by high fat feed than the home-made soybean milks.