| 应用PCR技术鉴定豆奶粉、奶粉及果汁饮料中的有效成分 |
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| 引用本文： | 陈文炳, 朱晓南, 邵碧英, 江树勋, 李寿崧. 应用PCR技术鉴定豆奶粉、奶粉及果汁饮料中的有效成分[J]. 中国食品学报, 2006, 6(1): 362-366 |
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| 作者姓名： | 陈文炳 朱晓南 邵碧英 江树勋 李寿崧 |
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| 作者单位： | 福建出入境检验检疫局检验检疫技术中心,福州,350001 |
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| 基金项目： | 国家质量监督检验检疫总局科研项目 |
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| 摘 要： | 应用PCR技术对日常生活中几种常见的与人类健康和安全有关的豆奶粉、奶粉及果汁饮料等加工食品中的有效成分,包括大豆凝聚素(Lectin)基因、牛线粒体基因、植物叶绿体rbcL基因以及真核生物中普遍存在的核糖体DNA(18SrDNA)片段等进行检测,建立了这些样品的DNA提取、纯化以及单PCR和二重PCR检测技术体系。结果表明:应用单PCR技术和二重PCR技术检测样品的结果一致,且稳定、重复性好。
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| 关 键 词： | 豆奶粉 奶粉 果汁饮料 PCR检测 |
| 文章编号： | 1009-7848(2006)01-0362-05 |
| 修稿时间： | 2005-09-12 |
Identification for Bovine and Some Plant Components in Soybean-milk Powder, Milk Powder and Fruit Juice by PCR |
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| Chen Wenbing,Zhu Xiaonan,Shao Biying,Jiang Shuxun,Li Shousong. Identification for Bovine and Some Plant Components in Soybean-milk Powder, Milk Powder and Fruit Juice by PCR[J]. Journal of Chinese Institute of Food Science and Technology, 2006, 6(1): 362-366 |
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| Authors: | Chen Wenbing Zhu Xiaonan Shao Biying Jiang Shuxun Li Shousong |
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| Abstract: | Bovine and soybean components in soybean- milk powder and milk powder, plant component in fruit juice were identified by PCR detection for mtDNA of bovine, soybean lectin gene, rbcL gene of plant chloroplast and 18S rD- NA. Duplex PCR of 18SrDNA and bovine, plant component (rbcL gene) respectively were also carried out. |
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| Keywords: | Soybean- milk powder Milk powder Fruit juice PCR detection |
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