| Aspergillus ficuum菊粉酶的分离纯化与鉴定 |
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| 引用本文： | 王静,金征宇,江波,徐学明.Aspergillus ficuum菊粉酶的分离纯化与鉴定[J].食品科学,2007,28(6):188-192. |
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| 作者姓名： | 王静 金征宇 江波 徐学明 |
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| 作者单位： | 江南大学食品学院； 江南大学食品学院 江苏无锡214036； 江苏无锡214036； |
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| 基金项目： | 国家自然科学基金;教育部科学技术研究重点项目 |
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| 摘 要： | Aspergillus ficuum菊粉酶系经硫酸铵分级沉淀、透析脱盐、DEAE-Cellulose离子交换色谱、Sepharose CL-6B凝胶过滤色谱和制备电泳技术分离纯化,获得五个电泳纯酶:三个外切菊粉酶组分Exo-I、Exo-II、Exo-III和两个内切菊粉酶组分Endo-I、Endo-II。SDS-PAGE分析测得五种酶的分子量分别为:Exo-I:69961Da,Exo-II:39973Da,Exo-III:45561Da,Endo-I:33574Da,Endo-II:30769Da。
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| 关 键 词： | 菊粉酶 分离纯化 鉴定 Aspergillus ficuum |
| 文章编号： | 1002-6630（2007）06-0188-05 |
| 修稿时间： | 2006-05-28 |
Purification and Identification of Aspergillus ficuum Inulinases |
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| WANG Jing,JIN Zheng-yu,JIANG Bo,XU Xue-ming.Purification and Identification of Aspergillus ficuum Inulinases[J].Food Science,2007,28(6):188-192. |
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| Authors: | WANG Jing JIN Zheng-yu JIANG Bo XU Xue-ming |
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| Affiliation: | School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China |
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| Abstract: | Aspergillus ficuum inulinases were purified in a sequence of operations including ammonium sulfate fractionation, ion exchange chromatography on DEAE-Cellulose, column chromatography with Sepharose CL-6B and preparative electrophoresis. Five purified enzymes were thus obtained, two having an endo-type activity and the other being exo-actings. The five enzymes were identified as Exo-I, Exo-II, Exo-III, Endo-I and Endo-II, with MW(molecular weight) of 69961Da, 39973Da, 45561Da, 33574Da, 30769Da, respectively, determined by SDS-PAGE. |
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| Keywords: | Aspergillus ficuum |
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