|
|||||
|
|
| 样品靶自动定位大气压基体辅助激光解析离子源的研制 | |
| 引用本文： | 张芳,王淑芳,郭长娟,粘慧青,周振.样品靶自动定位大气压基体辅助激光解析离子源的研制[J].现代仪器,2008,14(2):39-42. |
| 作者姓名： | 张芳 王淑芳 郭长娟 粘慧青 周振 |
| 作者单位： | 1. 中国科学院广州地球化学研究所,有机地球化学国家重点实验室,广州,510640;中国科学院研究生院,北京,100049 2. 长春师范学院,长春,130032 3. 中国科学院广州地球化学研究所,有机地球化学国家重点实验室,广州,510640;上海大学环化学院环境污染与健康研究所,上海,200072 |
| 基金项目： | 广东省科技攻关计划 , 广东省广州市科技攻关项目 |
| 摘 要： | 本文介绍实验室自制的样品靶可以自动定位的大气压基体辅助激光解析离子源(Atmospheric Pressure Matrix Assisted Laser Desorption/Ionization,AP-MALDI)的原理、结构,以及与飞行时间质谱仪(Time of flight Mass Spectrometer,TOFMS)联用对多肽样品的分析实验。所得到的生物大分子谱图表明:AP-MALDI离子源与高分辨TOFMS联用可以实现大气压下大分子高通量的精确质量检测,最低检测限可达2.5fmol。 |
| 关 键 词： | 大气压基体辅助激光解析离子源 飞行时间质谱仪 样品靶自动定位 |
The Study of automatic positioning sample target atmospheric pressure matrix assisted laser desorption/ionization |
|
| Zhang Fang,Wang Shufang,Guo Changjuan,Nian Huiqing,Zhou Zhen.The Study of automatic positioning sample target atmospheric pressure matrix assisted laser desorption/ionization[J].Modern Instruments,2008,14(2):39-42. | |
| Authors: | Zhang Fang Wang Shufang Guo Changjuan Nian Huiqing Zhou Zhen |
| Abstract: | This paper introduces the principle,configuration and primary experiment results of a home made automatic positioning sample target atmospheric pressure matrix assisted laser desorption/ionization (AP-MALDI)coupling with a home made time of flight mass spectrometer (TOFMS). The experiment results indicate that the AP-MALDI coupling with TOFMS can be used to detect large molecules with high accuracy and high throughput. The mass resolution of TOFMS can achieve as high as 8000(FWHM).A decent mass spectrum of peptide shows that the detection limit is 2.5 fmol. |
| Keywords: | Atmospheric pressure matrix-Assisted laser desorption/ ionization (AP-MALDI) Time of flight mass spectrometer(TOFMS) Automatic positioning sample target |
| 本文献已被 维普 万方数据 等数据库收录！ | |