The construction and cloning of synthetic genes coding for artificial proteins and expression studies to obtain fusion proteins |
| |
| Authors: | Biernat, Jacek Hasselmann, Hanne Hofer, Bernd Kennedy, Nick Koster, Hubert |
| |
| Affiliation: | BIOSYNTECH Biochemische Synthesetechnik GmbH Stresemannstrasse 268-280, D-2000 Hamburg 50 1Universitätskrankenhaus-Eppendorf, Dept of Immunology Martinistrasse, D-2000 Hamburg 2Gesellschaft für Biotechnologische Forschung mbH, Dept of Genetics Mascheroder Weg 1, 3300 Braunschweig 3Henkel KGaA, Dept of Biotechnology Postfach 1100, 4000 Düsseldorf 1 4Institute für Organische Chemie und Biochemie Martin-Luther-King-Platz 6, D-2000 Hamburg 13, FRG |
| |
| Abstract: | Synthetic genes coding for artificial proteins with predeflnedand nutritionally valuable amino acid compositions have beenconstructed and cloned In bacterial plasmid vector pKK233-2.The genes were constructed from three easily interchangeablecassettes encoding either essential, non-essentialor branched-chain amino acid residues. A potential hairpin loopstructure in the mRNA around the region of the ribosome bindingsite was probably the reason for blockage of translation fromthis vector. Two selected genes, AHB (containing one copy ofeach cassette) and A (consisting of six copies concatemerizedA6cassette) were cloned into pUR300, a (ß-Gal fusionvector and expressed as fusion proteins (ß-Gal-AHBand (ß-Gal-A6. |
| |
| Keywords: | artificial proteins/ fusion proteins/ gene expression/ messenger RNA structure/ synthetic genes |
| 本文献已被 Oxford 等数据库收录！ |
|
