Degumming rice bran oil using phospholipase‐A1 |
| |
Authors: | Sarode Manjula Anju Jose Soundar Divakar Rangaswamy Subramanian |
| |
Affiliation: | 1. Food Engineering Department, Central Food Technological Research Institute, Council of Scientific and Industrial Research, Mysore, IndiaPresent address: Department of Chemical Engineering, Adhiyamaan College of Engineering, Hosur – 635 109, India.;2. Food Engineering Department, Central Food Technological Research Institute, Council of Scientific and Industrial Research, Mysore, India;3. Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Council of Scientific and Industrial Research, Mysore, India |
| |
Abstract: | The efficacy of enzymatic degumming was assessed using the third generation phospholipase‐A1, Lecitase®‐Ultra (EC 3.1.1.3) from Thermomyces lanuginosa/Fusarium oxysporum with different qualities of crude rice bran oil. The phosphorus content in the oil reduced to ~10 mg/kg from an initial level of 390 mg/kg after 2 h of incubation period at 50°C. However, in the solvent‐phase degumming, there was practically no phospholipid reduction at lower water content (2%) due to the poor contact between the highly nonpolar solvent and the aqueous phase (citric acid, NaOH, and enzyme solutions). Increasing the water content to 20% reduced the phosphorus level in the degummed‐oil to 71 mg/kg but did not match the performance of oil‐phase degumming. The degumming efficiency of Lecitase®‐Ultra was effective in oil‐phase and suitable for practical application. Solvent‐phase enzymatic degumming offers more benefits but needs greater efforts to overcome the challenges. |
| |
Keywords: | Enzymatic degumming Membrane separation Phospholipase Phospholipid Rice bran oil |
|
|