Oxidation of Polycyclic Aromatic Hydrocarbons using Partially Purified Laccase from Residual Compost of Agaricus bisporus |
| |
| Authors: | K. Mayolo‐Deloisa C. Machín‐Ramírez M. Rito‐Palomares M. R. Trejo‐Hernández |
| |
| Affiliation: | 1. Center for Biotechnology Research, Autonomous University of Morelos State, Cuernavaca, Morelos, México;2. Center for Biotechnology‐FEMSA, Monterrey Institute of Technology, Campus Monterrey, Monterrey, México;3. Faculty of Chemical Sciences and Engineering, Autonomous University of Morelos State, Cuernavaca, Morelos, México |
| |
| Abstract: | Laccase partially purified from residual compost of Agaricus bisporus by an aqueous two‐phase system (Lac ATPS) was used in degrading polycyclic aromatic hydrocarbons: fluorene (Flu), phenanthrene (Phe), anthracene (Ant), benzo[a]pyrene (BaP), and benzo[a]anthracene (BaA). The capacity of the enzyme to oxidize polyaromatic compounds was compared to that of the crude laccase extract (CE). After treatment of 72 h, Lac ATPS and CE were not capable of oxidizing Flu and Phe, while Ant, BaP, and BaA were oxidized, resulting in percentages of oxidation of 11.2 ± 1, 26 ± 2, and 11.7 ± 4 % with CE, respectively. When Lac ATPS was used, the following percentages of oxidation were obtained: 11.4 ± 3 % for Ant, 34 ± 0.1 % for BaP, and 13.6 ± 2 % for BaA. The results reported here demonstrate the potential application of Lac ATPS for the oxidation of polycyclic aromatic hydrocarbons. |
| |
| Keywords: | Agaricus bisporus Aqueous two‐phase systems Laccase Oxidation Residual compost |
|
|
