色谱法检测真菌毒素的研究进展

色谱法在物质分离和检测方面应用广泛。真菌毒素在低浓度下就有明显毒性,采用色谱法可实现对真菌毒素的分离和微量检测。依据真菌毒素的自身特性采用不同的色谱方法进行检测,但不同的色谱法有不同的应用范围。薄层色谱法用于对真菌毒素的快速定性,气相色谱法需要对真菌毒素衍生化汽化后才可检测,液相色谱法对本身具有或衍生化后具有紫外和荧光吸收的真菌毒素才可检测,对无紫外和荧光吸收的则需质谱检测器。简述了色谱法在真菌毒素进行定性定量的研究情况,为利用色谱法对真菌素含量测定的实际运用和方法学开发提供参考。

Research progress in detection of mycotoxins by chromatography

Chromatography is widely used in components separation and detection. Since mycotoxins display toxicity even at low concentration, they can be separated and detected in trace by chromatography. Different chromatography methods were applied according to the characteristics of the mycotoxins. Thin layer chromatography (TLC) is suitable for rapid qualitative of mycotoxins. Gas chromatography (GC) prefers to detect the mycotoxins after derivatization and vaporization. Liquid chromatography (LC) is only applied to analyze the mycotoxins with or without derivatization which were of ultraviolet and fluorescence absorption, while for those without ultraviolet and fluorescence absorption use mass spectrometry detector. The features of the qualitative and quantitative analysis on mycotoxins were introduced, which provides a theoretical basis for the subsequent use of chromatographic method in application of detection on mycotoxins, especially synchronous detection of multiple mycotoxins in samples.