| 紫甘蓝总花色苷提取物抑制脂多糖诱导RAW264.7细胞的炎症反应 |
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| 引用本文： | 谭兵,庞琪期,钱波,王程强,曾榛,周燕园,宋家乐.紫甘蓝总花色苷提取物抑制脂多糖诱导RAW264.7细胞的炎症反应[J].现代食品科技,2018,34(11):8-14. |
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| 作者姓名： | 谭兵 庞琪期 钱波 王程强 曾榛 周燕园 宋家乐 |
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| 作者单位： | （1.桂林医学院基础医学院，广西桂林 541100）,(2.桂林医学院公共卫生学院，广西桂林 541100),(2.桂林医学院公共卫生学院，广西桂林 541100)(3.广西高校预防医学重点实验室，广西桂林 541100),(2.桂林医学院公共卫生学院，广西桂林 541100)(3.广西高校预防医学重点实验室，广西桂林 541100),(2.桂林医学院公共卫生学院，广西桂林 541100)(3.广西高校预防医学重点实验室，广西桂林 541100),(4.桂林医学院药学院，广西桂林 541100）,(2.桂林医学院公共卫生学院，广西桂林 541100)(3.广西高校预防医学重点实验室，广西桂林 541100) |
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| 基金项目： | 国家自然科学基金资助项目(81560530；81760589)；广西自然科学基金资助项目(2016GXNSFCA380026)；桂林医学院引进人才科研启动基金(04010150001)；2018年广西教育厅广西高等学校千名中青年骨干教师培育计划资助（桂教人[2018]18号） |
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| 摘 要： | 探讨紫甘蓝总花色苷提取物对脂多糖(LPS,1μg/mL)诱导RAW264.7细胞炎症反应的影响。以不同质量浓度(1μg/mL、10μg/mL、50μg/mL)的紫甘蓝总花色苷提取物处理RAW264.7细胞后,试剂盒法分别检测一氧化氮(NO)和前列腺素E2(PGE_2)的分泌水平。酶联免疫吸附法(ELISA)检测肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、IL-6和IL-8分泌水平。实时定量PCR(Quantitative real-time PCR,qRT-PCR)检测细胞中诱导型一氧化氮合酶(iNOS)、环氧合酶-2(COX-2)、TNF-α、IL-1β、IL-6和IL-8的m RNA表达水平。结果显示,紫甘蓝总花色苷提取物能有效下调iNOS与COX-2的m RNA表达,抑制NO和PGE_2的释放。特别地,高浓度的紫甘蓝总花色苷提取物(50μg/mL)能显著地抑制LPS所诱发的iNOS(65.80%)与COX-2(48.28%)的m RNA表达,降低NO(58.81%)和PGE_2(46.26%)的释放水平。同时,紫甘蓝总花色苷提取物还能显著下调TNF-α、IL-1β、IL-6和IL-8的m RNA表达水平,并抑制TNF-α、IL-1β、IL-6和IL-8的分泌。结果表明,紫甘蓝总花色苷提取物具有较强的抗炎活性,能够抑制LPS诱导的RAW264.7细胞所发生的炎症反应。
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| 关 键 词： | 紫甘蓝花色苷 RAW246.7细胞 炎症反应 细胞因子 |
| 收稿时间： | 2018/7/4 0:00:00 |
Inhibition of Lipopolysaccharide-induced RAW264.7 Cells Inflammatory Reaction by Total Anthocyanins from Red Cabbage |
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| TAN Bing,PANG Qi-qi,QIAN Bo,WANG Cheng-qiang,ZENG Zhen,ZHOU Yan-yuan and SONG Jia-le.Inhibition of Lipopolysaccharide-induced RAW264.7 Cells Inflammatory Reaction by Total Anthocyanins from Red Cabbage[J].Modern Food Science & Technology,2018,34(11):8-14. |
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| Authors: | TAN Bing PANG Qi-qi QIAN Bo WANG Cheng-qiang ZENG Zhen ZHOU Yan-yuan and SONG Jia-le |
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| Affiliation: | (1.School of Basic Medicine, Guilin Medical University, Guilin 541100, China),(2.School of Public Health, Guilin Medical University, Guilin 541100, China),(2.School of Public Health, Guilin Medical University, Guilin 541100, China) (3.Guangxi Colleges and University Key Laboratory of Preventive Medicine, Guilin Medical University, Guilin 541100, China),(2.School of Public Health, Guilin Medical University, Guilin 541100, China) (3.Guangxi Colleges and University Key Laboratory of Preventive Medicine, Guilin Medical University, Guilin 541100, China),(2.School of Public Health, Guilin Medical University, Guilin 541100, China) (3.Guangxi Colleges and University Key Laboratory of Preventive Medicine, Guilin Medical University, Guilin 541100, China),(4.School of Pharmacy, Guilin Medical University, Guilin 541100, China) and (2.School of Public Health, Guilin Medical University, Guilin 541100, China) (3.Guangxi Colleges and University Key Laboratory of Preventive Medicine, Guilin Medical University, Guilin 541100, China) |
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| Abstract: | |
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| Keywords: | red cabbage anthocyanins RAW246 7cell inflammatory reaction cytokines |
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