当归总黄酮提取方法比较及工艺条件优化

对当归总黄酮进行提取,比较其提取方法,优化其最适提取方法的工艺条件.试验分别采用水煎煮法、乙醇回流法、超声波提取法对当归总黄酮进行提取,采用亚硝酸钠-硝酸铝-氢氧化钠法测定总黄酮含量,采用正交试验优化提取工艺条件.试验结果表明：乙醇回流法为当归总黄酮的最适提取方法,当提取温度85℃、提取时间2.0h、料液比1∶30、乙醇浓度80％时,得当归总黄酮含量为10-066 mg·g-1,比优化前提高了59.52％.试验较为完整地对当归总黄酮的提取方法和提取条件进行了研究,可为开发和利用当归总黄酮提供理论依据.

A Comparison of the Extraction Methods of Total Flavonoids From Angelica Sinensis and the Optimization of Processing Conditions

The total flavonoids is extracted from Angelica sinensis, the extraction methods are compared, and the pro-cessing conditions of the optimum extraction method are optimized. Tests are carried out by using boiling water extractionmethod, ethanol refluxing method and ultrasonic extraction method to extract total flavonoids from Angelica sinensis, and theconcentration of total flavonids is determined by sodium nitrite-aluminum nitrate and sodium hydroxide method, then the pro-cessing conditions of extraction are optimized by orthogonal test. The test results show that the ethanol refluxing method canget the highest total flavonoids content from Angelica sinensis when extraction temperature is 85 ~C, extraction time is 2. 0 h,ratio of solid to liquid is 1 ： 30, alcohol concentration is 80% and the content of total flavonoids extracted from Angelicasinensis at this time is 10. 066 mg. mL-1 , which is 59.52% higher than that before optimization. The tests are studiedrelatively well on extraction method and extraction conditions of total flavonoids of Angelica sinensis, which can provide atheoretical basis for the development and utilization of the total flavonoids of Angelica sinensis.