1~100kDalton的胶原水解物的分子量分布测定的研究 |
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引用本文: | 刘闯,唐晋,肖文君,郭玉龙,刘善堂,彭必先. 1~100kDalton的胶原水解物的分子量分布测定的研究[J]. 明胶科学与技术, 2012, 0(3): 113-126 |
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作者姓名: | 刘闯 唐晋 肖文君 郭玉龙 刘善堂 彭必先 |
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作者单位: | 1. 武汉工程大学化工与制药学院,武汉,430073 2. University of Vienna, 1150 - Wien 3. 广西师范大学生命科学院,桂林,541004 4. 北京九州龙岭环保材料高新技术有限公司,北京,102209 5. 中国科学院理化技术研究所,北京,100080 |
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摘 要: | 采用凝胶板配方和电泳条件优化后的SDS-PAGE技术对酸法明胶水解物、碱法明胶水解物、胃蛋白酶法明胶水解物、胃蛋白酶法猪皮胶原水解物、明胶以及猪皮胶原蛋白透析物等进行表征,获得了将分布于一块凝胶板上的1~100kD的棒状胶原蛋白各组分的色谱图。胃蛋白酶法猪皮胶原蛋白水解物经电泳分离后的色谱图中,各谱带清晰明显,分子量均一,且均匀分布于1~100kD不等,这对棒状胶原蛋白分子的研究具有重要价值。
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关 键 词: | 十二烷基硫酸钠聚丙烯酰胺凝胶电泳 棒状胶原水解物(1~100kD) |
Four New Markers and New Molecular Sieve Formulations Adopted for Quantitative Characterisation of Rod - like Collagen Hydrolysates with Molecular Weight Distribution between 1 - 100 KDalton by SDS - PAGE |
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Affiliation: | Liu Chuang, Tang Jin, Xiao Wenjun, Guo Yulong, Lui Shantang, Peng Bixuan |
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Abstract: | An attempt was made to use CB - peptides as new makers and to optimise the gel formulas and electrophoresis conditions for SDS -polyaerylamide gel electrophoresis to char~erise the acid -hydrolyzed gelatin, .alkali -hydmlyzed gelatin, pepsinhydrolyzed gelatin as well as pepsin- hydrolyzed pig skin collagen. The different components of rod - like collagen hydrolyses with molecular weight possibly ranging from I to 100 kDalton can be separated, displayed clearly in their electrophoresis scanned chromatograms and quantitatively characterised successfizlly. In a typical electrophoresls chroraatogram d pepsin hydrolyzed pig skin collagen, all the bands were dearly localised and their molecular weights orderly were shon to be dislributed between 1 and 100 kD after calibrating. All these are of important values foz the test of collagen hydrolyses. |
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Keywords: | SDS - PAGE rod - like hydealyzed conagens ( 1 - 100kD) |
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