气质联用技术研究爱国者蓝莓不同溶剂提取物

目的 对比不同溶剂对蓝莓果化合物提取的异同。方法 本研究使用4种不同的溶剂提取爱国者蓝莓化合物, 分别为: S1(100%甲醇和0.1%的甲酸), S2(50%甲醇水溶液), S3(70%丙酮、29.5%水、0.5%乙酸)和S4(50%乙醇), 并结合柱前衍生化-气相色谱质谱联用(GC-MS)技术检测不同溶剂蓝莓提取物。结果 研究结果表明, 蓝莓果中含有酸、醇、酚、酯、糖、酮、烷烃等类化合物; 同时研究结果还发现, S4适合提取酸类化合物, S3适合提取糖类化合物, S2相比其他提取溶剂, 能提供更多关于酯类、酚类、烷烃类和其他化合物信息, 因此, S2是研究蓝莓化合物组分较优的提取剂。结论 本实验对于不同类别蓝莓组分的需求提供了研究的新思路, 同时对蓝莓品质控制和功能性成分的高效利用有非常重要的意义。

Analysis of chemical compounds in different solvent extracts with gas chromatography-mass spectrometry

Objective To compare similarities and differences of different solvents in the extracts of blueberry fruit. Methods Compounds in the extracts of Patriot blueberry (Semen trigonellae) extracted using different solvents of S1 (100% methanol with 0.1% formic acid), S2 (50% aqueous methanol), S3 (70% acetone, 29.5% water, with 0.5 % acetic acid) or S4 (50% aqueous ethanol) were analyzed and compared by gas chromatography-mass spectrometry (GC-MS) with pre-column derivatization. Results Acid, alcohol, phenolic compounds, esters, sugar, ketone, alkane and other compounds were identified from the extracts. The results showed that S4 was the more suitable solvent for extracting the acids and S3 for extracting sugars while S2 was relatively good in extracting ester, phenolic compounds, alkane and others compounds from the fruits. So S2 solution was a better choice than the others for extracting chemical compounds from blueberry. Conclusion This study provides a new insight into the needs of different types of blueberry components. Meanwhile, this overall identification of chemical components in blueberry is of great significance to the quality control and effective utilization of its functional compounds.