Application of nanostructured ZnO films for electrochemical DNA biosensor |
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| Authors: | Maumita Das Gajjala Sumana R Nagarajan BD Malhotra |
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| Affiliation: | 1. Department of Biotechnology, University of Kashmir, Srinagar, India;2. Center of Excellence in Nanotechnology and Chemistry Department, King Fahd University enter of Petroleum and Minerals, Dhahran, Saudi Arabia |
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| Abstract: | Nanostructured zinc oxide (nsZnO) films have been fabricated onto conducting indium–tin–oxide (ITO) coated glass plate, by cathodic electro-deposition to immobilize probe DNA specific to M. tuberculosis via physisorption based on strong electrostatic interactions between positively charged ZnO (isoelectric point = 9.5) and negatively charged DNA to detect its complementary target. Electrochemical studies reveal that the presence of nano-structured ZnO results in increased electro-active surface area for loading of DNA molecules. The DNA–nsZnO/ITO bioelectrode exhibits interesting characteristics such as detection range of 1 × 10?6 ? 1 × 10?12 M, detection limit of 1 × 10?12 M (complementary target) and 1 × 10?13 M (genomic DNA), reusability of about 10 times, response time of 60s and stability of up to 4 months when kept at 4°C. |
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