Preparation of kanamycin powder by an optimized spray freeze-drying method |
| |
| Authors: | Jae-Young Her Chi-Sung Song Seung Ju Lee Kwang-Geun Lee |
| |
| Affiliation: | 1. Department of Food Science and Technology, Dongguk University-Seoul, Pil-dong, Jung-gu, Seoul 100715, South Korea;2. Korea Institute of Machinery & Materials, Jang-dong, Yusung, Daejeon 305-343, South Korea;1. Laboratoire d’Histologie et de cytogénétique et maladie de l’enfant UR12ES10, Faculté de Médecine, Université de Monastir, Tunisia;3. Mountain Research Centre (CIMO) ESA, Polytechnic Institute of Bragança, Campus de Santa Apolónia, 1172, 5301-855 Bragança, Portugal;2. Laboratoire de recherche Bioressources : Biologie Intégrative & Valorisation, Institut supérieur de biotechnologie, Université de Monastir, Tunisia;1. Instituto de Biotecnología (IBT), Universidad Nacional Agraria La Molina-UNALM, Av., La Molina s/n, Lima, Peru;2. Institut des Sciences de la Vie, UCLouvain, Croix du Sud 2/8, B-1348 Louvain-la Neuve, Belgium;3. Food & Biobased Research, Wageningen UR, Bornse Weilanden 9, Wageningen 6708WG, The Netherlands;1. Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, 56000, Kuala Lumpur, Malaysia;2. Faculty of Engineering, Technology and Built Environment, UCSI University, 56000, Kuala Lumpur, Malaysia;3. Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia |
| |
| Abstract: | The physical and antibiotic properties of kanamycin powders obtained by spray freeze drying (SFD) were compared with those of raw kanamycin. The SFD procedures were optimized to prepare kanamycin for use as an inhaled drug. Scanning electron microscopy (SEM) and a laser particle size analyzer were applied to estimate physical structure and properties of the particle. In addition, the disk diffusion method was used to compare the antibiotic activity of raw kanamycin and that produced by SFD. According to SEM, the kanamycin particles had various sizes and shapes with porous structures at different SFD conditions. The diameters of the kanamycin powders were between 13.5 μm and 21.8 μm, and their aerodynamic particle sizes were between 3.58 μm and 6.39 μm. The antibiotic activities of the raw and spray freeze-dried kanamycin samples were not significantly different (P > 0.05). The optimized conditions for annealing temperature, annealing time, kanamycin concentration, pressure, and nozzle tip lift were ? 15 °C, 5 h, 10% kanamycin, 100 kPa, and, 1 mm, respectively. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
